The manager told the club website: “The players who were not available for the Arsenal game – Dalot, Martial, Sancho, are also not available for tomorrow.”
Dalot and Martial are absent with injuries, with the right-back exiting the quarter-final against Charlton after just 40 minutes.
Trio ruled out
Jadon Sancho is the closest of the three players. He is in first team training, having returned last week as he continues to build up his fitness.
Ten Hag does still not believe he is ready for first team action, taking a patient approach with the former Borussia Dortmund star.
It might not be too long until he makes his return, however, the semi-final first leg comes a little too soon.
At right-back, Dalot’s absence will mean another start for Aaron Wan-Bissaka at the position.
Martial not being fit was not unexpected. No return date has been set for his return. Wout Weghorst could continue in attack, but he is not the only option.
Marcus Rashford could lead the line, supported by any of Alejandro Garnacho, Antony or even Facundo Pellistri.
The first leg takes place at the City Ground, before United face Reading in the FA Cup at Old Trafford at the weekend, and then the home leg against Nottingham Forest next week, as United look to book a place in the Carabao Cup final.